Bio-Analytical Separation Science Group

Sebastiaan Eeltink

Research Highlights

Our research focusses on the design, development, and application of novel analytics for high-resolution profiling of biomolecules and biomolecular interactions using ultra-high-pressure liquid chromatography (UHPLC) and multi-dimensional (2D and 3D) liquid-chromatographic workflows with mass-spectrometry (MS) detection.

The focus is directed towards the development of and application of emerging column technologies to establish bioanalytical separations with unprecedented resolving power. We design novel column structures and chemistries for separations and well as catalyzed reactions. In particular, we focus on polymer monolithic materials.

Novel concepts for multi-dimensional 2D-LC and 3D-LC separations are pursued via microfluidic solutions. We utilize state-of-the-art microfabrication techniques to develop thermoplastic chips with the desired microchannel layout, allowing an enormous versatility in our separation methodologies.

UHPLC-MS and LCxLC-MS method development is conducted in support of post-genomic biotechnology research and medical diagnostics. Our focus is directed to the analysis of highly complex biological samples, which constitutes an enormous number of analytes with a large dynamic range in concentration. Additionally, we target the analysis of emerging biotherapeutics.

The Eeltink Lab also manages the VUB proteomics platform which offers high-performance LC-MS proteomics profiling expertise and services to academic and industrial collaborators.




  • Manuela Mafosso Siekapen (Assessment of external band broadening of UHPLC systems and its effect on separation efficiency) and Matthew Scott Wilmot (Development and characterisation of immobilised enzymatic reactors for bottom-up proteomics) have succesfully defended their MSc thesis.